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• Frequently Asked Questions

• How do we interpret CLIP-seq data with RBP–RBP interactions?

  We apply a soft-clustering method, RBPgroup, to various CLIP-seq datasets to group together RBPs that specifically bind the same RNA sites. Such combinatorial clustering of RBPs helps interpret CLIP-seq data and suggests functional RNA regulatory elements. Furthermore, we validate two RBP–RBP interactions in cell lines.

• What does pro seq stand for?

  Precision Nuclear Run-on Sequencing. Precision nuclear run-on sequencing (PRO-Seq) maps RNAPII pause sites with base-pair resolution during RNA transcription (Kwak et al., 2013) (Mahat et al., 2016). This approach is similar to GRO-Seq, but it provides the added benefit of single-base resolution. RNAPII initiation sites can be mapped using ...

• What can propro seq do for you?

  PRO-seq (Precision Run-On Sequencing) offers single nucleotide resolution of nascent RNA 3’ ends. This information can be used to identify candidate enhancer RNAs and profile the immediate transcriptional effects of experimental perturbations such as drug-treatment, cell differentiation, or protein degradation.

• What is preprecision nuclear run on sequencing pro seq?

  Precision nuclear run-on sequencing (PRO-Seq) maps RNAPII pause sites with base-pair resolution during RNA transcription (Kwak et al., 2013) (Mahat et al., 2016). This approach is similar to GRO-Seq, but it provides the added benefit of single-base resolution.

• Is the active motif ATAC-seq kit reproducible?

  The Active Motif ATAC-Seq Kit generates high-quality data. ATAC-Seq data from experiments performed with the ATAC-Seq Kit correlates very well with published ATAC-Seq data, highlighting that this assay is robust and reproducible. Figure 2: ATAC-Seq Kit Libraries Correlation with Published ATAC-Seq Dataset.